Hence, they have both physiological and pathophysiological roles in biology 1, 2, 3, 4. Reactive oxygen species (ROS) (Box 1) are intimately involved in redox signalling but in some situations can also lead to oxidative damage. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. Nature Metabolism volume 4, pages 651–662 ( 2022) Cite this article Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo

Ros reactive oxygen